Polymorphism in the APNH gene, detected by denaturing gradient gel electrophoresis

Polymorphism in the APNH gene, detected by denaturing gradient gel electrophoresis.

Description: A 376 bp genomic DNA fragment corresponding to nucleotides 2 5 — 351 of the published cDNA coding sequence for the Na + /H+ antiport gene, APNH (1), was amplified by PCR using 5'-CGAGGCTGGCTCTGGAAGCAGCACCATG-3' and 5'-TATCTTCATGAGGCAGGCCAGAAGGAT-3' as the 5' and 3' primers respectively. PCR was performed with a programmable thermal cycler (MJ Research Inc.) using the following cond...

Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR produ...

Denaturing Gradient Gel Electrophoresis Detected by Genus-Specific PCR and Bifidobacterial Diversity in Human Feces

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

Study of Bacterial Diversity in Poultry Gut Using Denaturing Gradient Gel Electrophoresis

Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...